Esmaspäeval, 26. nov kell 14.15 toimub Physicumis W.Ostwaldi 1 seminariruumis D312
Laserspektroskoopia seminar No 264
Esineb Dr Glen Kelp
Department of Physics, The University of Texas at Austin, Austin, TX 78712, USA
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA
Metasurfaces for Cell Capturing and Infrared Spectroscopy
Metasurfaces, a 2-dimensional subclass of metamaterials, have attracted attention due to the ability to fabricate thin devices with extraordinary optical properties /1/, enabling variety of applications: beam shaping, polarization control, light emission and absorption, and sensing. High localized electric field enhancement of mid-infrared (IR) Fano resonant metasurfaces makes them an appealing platform for sensing of biological material.
This talk will introduce metasurface-enhanced infrared spectroscopy (MEIRS) using plasmonic metasurfaces to study mid-IR spectra of human cells, a methodology known as spectral cytopathology /2/. Various characteristic mid-IR vibrational modes of cells are recognized using MEIRS of fixed and live cells. Combining microfluidics, dielectrophoresis (DEP) and MEIRS, a powerful device for live cell sorting and spectral analysis is created, with potential applications in clinical spectral cytopathology. Distinguishing of fixed normal and cancerous colon cells, as well as live epithelial cancer and blood cells by their mid-IR spectra using principal component analysis (PCA) is demonstrated. Live DEP captured cell spectra can be measured very fast (within a few minutes) and due to disparate dielectric properties of epithelial and blood cell types, only epithelial cells can be captured onto the metasurfaces using DEP, while preventing blood cells from being collected. This opens the possibility to use such a device for selective capturing of circulating tumor cells from blood samples for immediate spectral characterization.
/1/ C Wu, N Arju, G Kelp, … Spectrally selective chiral silicon metasurfaces based on infrared Fano resonances, Nat Commun 2014
/2/ N Arju, SD Gupta, G Kelp, … Metasurface-Enhanced Infrared Spectroscopy: From Protein Detection to Cells Differentiation, Optical Sensors, 2016
Kõikide huviliste osavõtt oodatud!
Teate edastab I. Sildos, 22 november 2018 a.