Esmaspäeval, 14. mail 2018 kell 14.15 toimub Physicumis W.Ostwaldi 1 seminariruumis D312
Laserspektroskoopia ja biofüüsika ühisseminar No 254
Esineb: A.S. Gur’ev1, 2, 3
1M.F.Vladimirsky Moscow Regional Clinical and Research Institute (MONIKI), Moscow, Russian Federation
2Medtechnopark Ltd., Moscow, Russian Federation
3 External post-graduate, Laboratory of Laser Spectroscopy, Institute of Physics, University of Tartu
Coherent fluctuating nephelometry application in laboratory practice. Material of PhD thesis (physics)
Coherent fluctuation nephelometry (CFN) is a new optical method used to measure the turbidity of liquid samples with high sensitivity. In CFN the fluctuations of intensity of scattered light are detected, it gives several advantages in comparison with traditional nephelometry. One of the advantages is weak influence of cuvette optical quality on sensitivity limit. CFN is shown to be effective in laboratory practice; in particular high sensitivity of 103-104 bacteria/ml is usefull in clinical laboratory microbiology for solving two main problems – rapid antibiotic susceptibility testing and urine samples screening for bacteriuria. More than 250 antibiotic susceptibility tests were made; the agreement with classical methods was from 84% to 88%. More than than 650 urine samples were analyzed; high sensitivity and specificity were shown (up to 95.2% and 96.9%) in comparison with classical methods. The effictiveness of CFN-analyzer and Sysmex UF-1000i (flow cytometry) were compared for bacteriuria screening, the superior diagnostic efficiency of CFN was shown.